Serological and Molecular Characterisation of Virus Infecting Watermelon (Citrullus lanatus) in Adim-Biase, Cross River State, Nigeria
O. I. Eyong, E. E. Ekpiken, G. M. Ubi, A. O. Alobi
Annual Research & Review in Biology · pp. 66–72 · Published 14 Nov 2020
10.9734/arrb/2020/v35i1130300Abstract
Watermelon, Citrullus lanatus, (Thunb.) is an annual crop in the Cucurbitaceae family grown for it fruits. It nutritional and medical uses helps in combating some illnesses and maintaining good health. The production of this crop in Nigeria is seriously under threat by virus disease. A survey of some farms in Adim, Biase Local Government Area of Cross River State, Nigeria during the 2019 planting season revealed mosaic and chlorotic symptoms. Mechanical inoculation was performed on young seedlings of Cucumeropsis mannii from watermelon samples showing viral symptoms and latter tested against the universal potyvirus antiserum and further detected by RT-PCR assay using cylindrical inclusion (CI) primer. The amplified cDNA was cloned and the nucleotide sequence determined. The serology result showed that there was positive reaction against the potyvirus antiserum confirming it to be a potyvirus. The gene sequence analysis revealed 86% sequence identity with Algerian watermelon mosaic virus. This is the first report of Algerian watermelon mosaic virus strain infecting watermelon in Nigeria.
Cited by 0
No indexed citations yet.
Related research
- Screening for Hepatitis B Virus among HIV Infected Women Seeking Antiretroviral Therapy at National Institute for Pharmaceutical Research and Development, Abuja, Nigeria — shares topic coverage
- Urinary Pathogens amongst Parturients at the University of Port Harcourt Teaching Hospital, South-South, Nigeria — shares topic coverage
- Risk Factors of Hypertension among Type 2 Diabetic Patients in Imo state, South East of Nigeria — shares topic coverage
- Willingness to Participate and Pay into a Community- Based Health Insurance Scheme in Imesi-Ile, a Rural Community in Osun State, Nigeria — shares topic coverage
- Prevalence and Comorbidity of Depression and Anxiety among Medical Students in Babcock University, Ogun State, Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.