Evaluation of Antiradical Effect and Dosage of Total Polyphénols of Extracts Abrus prectorius Leaves a Plant Used in the Treatment of Diabetes in Côte d’Ivoire

Abstract

Aims: The aim of this study was to evaluate the antiradical effect of extracts aqueous and ethanolic of Abrus precatorius Leaves and to determine the polyphenols. Place and Duration of Study: Microbiology and Biotechnology Laboratory, Ecology Research Center, Nangui Abrogoua University, BP 109, Abidjan 08, Côte d'Ivoire. The first part of this study was carried out from November 28, 2024 to December 31, 2024; the second part was conducted from January 18, 2025 to Mars 13, 2025. Methodology: The anti-radical effect was determined by scavenging DPPH free radical in the presence of control (Vitamin C) and inhibition concentration 50 (IC50) of each extract was determined. The polyphenols were dosed using the Folin-Ciocalteu reagent. Results: All extracts exhibited marked antiradical effect by scavenging DPPH free radical in a concentration dependent manner. There were recorded IC50 values of 0.813 mg/ml for aqueous extract and 0.283 mg/ml for ethnolic extract which are different from Vitamin C (7.10-3 mg/mL). Aqueous and ethanolic extracts of A. precatorius leaves are rich in polyphenols with levels of 178.34 ± 1.67 mg GAE/g and 163,28 ± 1,31 mg GAE/g respectively. The extracts of A precatorius show anti-radical effect in DPPH test with good polyphenol content, characterized by high levels of flavonoids (93.44 ± 1.83 mg QE/g) and condensed tannins (16,31 ± 0,24 mg CE/g) with aqueous extract. Conclusion: The antiradical effect observed with extracts of A. precatorius leaves rich in polyphenols could justify its antioxidant activity which would be at the origin of the antidiabetic properties attributed to the plant.