Dual Wavelength and First Order Derivative Spectrophotometric Methods for Simultaneous Estimation of Rivaroxaban and Aspirin in Synthetic Mixture

Abstract

Two simple, sensitive, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of binary mixture of Rivaroxaban and Aspirin in their pure form and synthetic mixture in difficult ratio 1:20(Rivaroxaban: Aspirin). The first method is the dual wavelength method, where 250 nm and 286.44 nm were selected as λ1 and λ2 for determination. And the two wave length for determination of Aspirin were 243.53 nm and 259.2 nm. The linearity range was studied over concentration ranges (2 – 12) μg/mL and (40-240) μg/mL for Rivaroxaban and Aspirin respectively in both methods, with correlation coefficients not less than 0.9996 and up to 0.9999 respectively in a row. The limit of detection (LOD) and limit of quantification (LOQ) were 0.24μg/mL and 0.82 μg/mL for Rivaroxaban and for Aspirin were7.03 μg/mL and 22.01 μg/mL respectively in the first method. The second method is First order derivative. The wavelengths 275.25 nm and 250 nm were selected as zero crossing point to determine Rivaroxaban and Aspirin in a row. The LOD and LOQ were 0.31 μg/mL and 0.95 μg/mL for Rivaroxaban; and for Aspirin was found to be7.13 μg/Ml and 21.60μg/mL respectively. The two methods were successfully validated as per ICH guidelines. Both methods were simple, sensitive and rapid.