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Research Article Open access CC BY 4.0

Purification and Characterization of Superoxide Dismutase Enzyme from Punica granatum L.

Caglar Ozdemir, Nuri Gulesci, Ramazan Bilgin

Asian Journal of Research in Biochemistry · pp. 30–35 · Published 14 Dec 2021

10.9734/ajrb/2021/v9i330203

Abstract

Superoxide dismutase (SOD) constitutes a very important antioxidant defense against oxidative stress in the body. SOD is found aplenty in many organisms, from microorganisms to plants and animals. Pomegranate (Punica granatum L.) peels and fruits have been used in traditional medicine. In this study, SOD (E.C 1.15.1.1) was purified from Punica granatum L. For this purpose, Punica granatum L. was homogenized centrifuged, fractioned with ammonium sulfate to precipitate, and then DEAE chromatography separation was applied.  Punica granatum L. was purified 16.60-fold. SOD enzyme-specific activity was found as 166 U/mg protein.

Punica granatum L. pomegranate purification superoxide dismutase

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