Detection of Salmonella spp. and Microbiological Analysis of Milk and Milk Based Products Available within Dhaka Metropolis, Bangladesh
Sharmina Yasmin, Shahana Parveen, Md. Sakil Munna, Rashed Noor
Microbiology Research Journal International · pp. 474–480 · Published 8 Dec 2014
10.9734/BMRJ/2015/11010Abstract
Aims: With a previous observation of pathogenic drug-resistant microorganisms in raw milk samples, current investigation further endeavored to determine the presence of Salmonella spp. and portrayed a complete map of the overall microbiological feature of milk and milk based products available within Dhaka metropolis, Bangladesh. Methodology: Conventional cultural and biochemical methods were carried out to isolate and enumerate the milk and milk based products accessing microorganisms. Standard agar disc diffusion method was conducted to assess the anti-bacterial resistance pattern of the isolated Salmonella spp. Results: Out of 35 samples studied, 16 were found to be contaminated with E. coli (~106 cfu/ml) and 9 samples were found to be contaminated with Salmonella spp. (~105 cfu/ml). Fungal growth was also observed in all samples (~106). The Salmonella isolates were found to be resistant against ampicillin (10 µg), cotrimoxazole (25 µg), chloramphenicol (30 µg), ciprofloxacin (5 µg), cefixime (5 µg) and sensitive against ofloxacin (5 µg). Conclusion: As projected from the current study, the proliferation of Salmonella spp. along with their drug-resistance traits could impart the morbidity and mortality associated risk to the overall public health safety.
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