Detection of Male Infidelity through Biochemical Analysis of Seminal Related Enzymes Prostate Specific Antigen and Acid Phosphatase

Abstract

Background: This study investigated the use of seminal-related enzymes, Prostate Specific Antigen (PSA) and Acid Phosphatase (AP), for detecting semen on male underwear as a potential method for identifying male infidelity. Objective: The primary aim was to assess the effectiveness of PSA and AP enzymes in detecting semen stains on various male underwear after different conditions of use. Methods: The research involved four male volunteers from different parts of Bayelsa, categorized into four groups: Group A, Group B, Group C, and Group D. Each participant was given brand-new male underwear. Group A served as the control and did not engage in sexual activity, he wore his boxers and returned it. Group B wore their boxers immediately after engaging in sexual intercourse and returned it. Group C took a bath after sexual activity, wore their boxers, washed them before returning them. Group D took a bath after sexual activity, wore their boxers, but did not wash them before returning them. The underwear from all groups were tested for the presence of semen using PSA and AP enzyme assays. Results: Data were expressed as mean±SD, and statistical significance was determined at a 95% confidence interval, with significance defined as p ≤ 0.05. The results showed no statistically significant difference in enzyme concentrations among the four groups. However, Group B exhibited the highest Acid Phosphatase (ACP) concentration, while Group A had the lowest. The highest PSA concentration was found in Groups C and D, with the lowest concentration in Group A. Conclusion: This study concluded that both ACP and PSA enzymes are not highly effective methods for detecting male infidelity. However, the PSA test proved to be more reliable for detecting seminal stains on fabric, particularly after different washing treatments. These findings could help guide the selection of the appropriate method for detecting semen stains, especially when the amount of semen is low or the fabric has been washed.