In vitro Regeneration of Corchorus olitorius var. O-4
Tania Afroz, Md. Mahabub Alam, Md. Ekramul Hoque
Asian Journal of Advances in Agricultural Research · pp. 32–38 · Published 16 Jan 2025
10.9734/ajaar/2025/v25i1578Abstract
The aim of this study was to determine how several plant growth regulators, such as 6-benzylaminopurine (BAP), indole acetic acid (IAA), and indole-3-butyric acid (IBA), affected the tossa jute plant's ability to regenerate in vitro. However, the most calluses and shoots were produced when the cotyledon-attached petioles of Corchorus olitorius var. O-4 were utilized as explants and inoculated in Murashige Skoog (MS) media supplemented with 2.5 mg/L BA + 0.5 mg/L IAA. Two weeks after induction (WAI), the 2.5 mg/L BA + 0.5 mg/L IAA treatment generated five leaves. The IBA therapy at 2.0 mg/L resulted in the regeneration of the majority of roots. The treatment 0.6 mg/L IBA produced the longest roots and the highest proportion of root induction. Regenerated plantlets have a 68.4% survival rate in shaded conditions and an 87.2% survival rate in full sunlight. Thus, in order to genetically alter the organism, an effective technique for Corchorus olitorius var. O-4 in vitro regeneration has been established.
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