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Research Article Open access CC BY 4.0

Orthogonal Test Design to Optimize on Ammonium Removal by Pseudomonas stutzeri ZH-1

Caifang Wen, Qingping Hu

Journal of Applied Life Sciences International · pp. 1–7 · Published 3 Feb 2017

10.9734/JALSI/2017/31281

Abstract

To give reference for the application of nitrobacteria in actual wastewater treatment, the impact factors of C/N ratio, shaking speed (DO) and carbon source on ammonium removal by Pseudomonas stutzeri ZH-1 was investigated. Through orthogonal test, the optimal conditions for NH4+-N removal were C/N 12, shaking speed 100 r/min and sodium citrate as carbon source. The strain ZH-1 exhibited efficient nitrifying ability and maximum NH4+-N removal efficiencies were 99.66%, and removal rate was 4.27 mg L-1h-1. The results demonstrated that C/N and DO were the most important factors influencing the performance of heterotrophic nitrification. Also, these findings stated that the strain ZH-1 might be an effective and suitable candidate for ammonium nitrogen removal in industrial wastewater treatment.

Orthogonal test ammonium nitrogen optimization Pseudomonas stutzeri

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