Molecular Detection of Transgenes in Improved Varieties of Cowpea
Kingsley Onuh, Karderam Bukar Dikwa, Victoria Moltong Dan, Mohammed Sani Abdulsalami, Ugochukwu Okechukwu Ozojiofor, Enimie Endurance Oaikhena, Kenneth Onuh, Ebipade Kereakede, Asabe Danjuma
International Journal of Biochemistry Research & Review · pp. 1–9 · Published 29 Aug 2022
10.9734/ijbcrr/2022/v31i830340Abstract
Cowpea is one of the most consumed food in Africa. Recombinant DNA technology has been used to insert foreign genes into plants genome via cassettes there by creating a new generation of plants with desired traits. Such plants possess improved seed quality, yield and resistance to pests and pathogens. The aim of this study is to screen for transgene in Cowpeas sold in Nigerian markets using molecular analysis. Five Improved varieties of Cowpea were obtained from IITA while local varieties were purchased from Terminus Market in Jos. All samples were screened for the presence of transgene via DNA extraction and PCR. Results shows the presence of transgene in MKT1, IT89KD and IT99KD, CAMV, NOS and VIP3BA respectively and none was found in local varieties.
Cited by 1
Ugochukwu Ozojiofor · Journal of Current Biomedical Research · 2023
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