Determination of Polyphenols, Reducing Potential and FT-IR Analysis of Green Tea (Camellia sinensis L.) Leaves Extract for Nanoparticles Synthesis
Komal Bisht, Niharika Pandey, Sneh Gautam, Pushpa Lohani
Journal of Advances in Biology & Biotechnology · pp. 842–850 · Published 2 Aug 2024
10.9734/jabb/2024/v27i81206Abstract
Green Tea (Camellia sinensis L.) is renowned for its therapeutic properties due to its phenolic compounds. Extracts from plants with high polyphenol content possess antioxidant properties that neutralize free radicals involved in the oxidation of various molecules. This study investigated the total phenolic and flavonoid content, 1,1-diphenylpicrylhydrazyl (DPPH) radical scavenging activity (RSA), and ferric reducing antioxidant power (FRAP) of commercially available green tea leaves for potential use in the green synthesis of silica nanoparticles. The antioxidant activity of green tea leaves demonstrates its reducing power, which is crucial for utilizing its extract in nanoparticle synthesis. Gallic acid and quercetin served as standards in the Folin–Ciocalteu and aluminum chloride methods, respectively, to measure total phenolic and flavonoid concentrations. To determine antioxidant content ascorbic acid and gallic acid were standards for the DPPH free radical scavenging and FRAP assays. Fourier Transform Infrared (FT-IR) spectra was performed to evaluate the green tea extract. The results revealed green tea leaves exhibited high phenolic, flavonoid content and significant antioxidant activity. The presence of few notable vibrational bands in FT-IR graph also confirmed the existence of chemical substances that are associated with antioxidant qualities (polyphenols, flavonoids). Indicating that the green tea leaf extract can act as an effective reducing and capping agent in the synthesis of silica nanoparticles.
References (22)
- 1 How to Read and Interpret FTIR Spectroscope of Organic Material [DOI]
- 2 Tea Catechins and Polyphenols: Health Effects, Metabolism, and Antioxidant Functions [DOI]
- 3 Rapid biological synthesis of silver nanoparticles using plant leaf extracts [DOI]
- 4 The Mechanism of Antioxidant Action in Vitro [DOI]
- 5 Tansy fruit mediated greener synthesis of silver and gold nanoparticles [DOI]
- 6 Antioxidant and antimicrobial activities of tea infusions [DOI]
- 7 Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes [DOI]
- 8 Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia [DOI]
- 9 Effect of drying method on the antioxidant capacity of six Lamiaceae herbs [DOI]
- 10 Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: Phytochemical, antioxidant and in vitro cytotoxicity studies [DOI]
- 11 Eco-Friendly Greener Synthesis of Nanoparticles [DOI]
- 12 Biosynthesis of silver nanoparticles using murraya koenigii (curry leaf): An investigation on the effect of broth concentration in reduction mechanism and particle size [DOI]
- 13 Evaluation of drying methods with respect to drying kinetics, mineral content and colour characteristics of rosemary leaves [DOI]
- 14 Green Synthesis of Iron Nanoparticles Using Green Tea leaves Extract [DOI]
- 15 Antioxidant properties and color parameters of herbal teas in China [DOI]
- 16 Green Synthesis of Silver Nanoparticles: Optimizing Green Tea Leaf Extraction for Enhanced Physicochemical Properties [DOI]
- 17 Antioxidant activity, ascorbic acid, phenolic compounds and sugars of wild and commercial Tuberaria lignosa samples: Effects of drying and oral preparation methods [DOI]
- 18 Determination of Antioxidant Activity in Tea Extracts, and Their Total Antioxidant Content [DOI]
- 19 Synthesis and Characterization of Graphene Oxide Derivatives via Functionalization Reaction with Hexamethylene Diisocyanate [DOI]
- 20 Manipulation of the Phenolic Quality of Assam Green Tea through Thermal Regulation and Utilization of Microwave and Ultrasonic Extraction Techniques [DOI]
- 21 Evaluation of in vitro antioxidant potential of Tea (Camelia sinensis) leaves obtained from different heights of Darjeeling Hill, West Bengal [DOI]
- 22 Total phenolic and flavonoid compound of crude and purified extract of green tea leaves (Camellia sinensis) from Makassar-Indonesia [DOI]
Cited by 11
Meng Zhang, Zhiyu Qi, Bowen You · Journal of Environmental Chemical Engineering · 2025
Amri Amri, Najma Annuria Fithri, Muhammad Said · Bulletin of Chemical Reaction Engineering & Catalysis · 2025
Mlungisi F. Mngoma, Lembe S. Magwaza, Asanda Mditshwa · South African Journal of Botany · 2025
Rand G. Al-Baghdady, Bashar M. Thejer, Yasser M. Taay · Cell Biochemistry and Biophysics · 2025
Showing 4 of 11 known citations — external sources report more than can currently be individually listed.
Related research
- Infrared Spectroscopic Analysis of Urinary Calculi: A Retrospective Study in Argentinean Patients — shares topic coverage
- Phytochemical Analysis, Antimicrobial and Antioxidant Activity of Clitoria ternatea Blue and White Flowered Leaves — shares topic coverage
- A Comparative Study of in vivo Plant and in vitro Callus Extracts of Centratherum punctatum Cass — shares topic coverage
- Structural Characterization Using FT-IR and NMR of Newly Synthesized 1,3-bis(3-formylphenoxymethyl)-2,4,5,6-tetrachlorobenzene and 1,3-bis(3-(2-hydroxyphenyliminomethyl)phenoxymethyl)-2,4,5,6-tetrachlorobenzene — shares topic coverage
- Application of a Multi-Analytical Toolset to Characterize the Ancient Pottery from Kottapalayam, Tamilnadu, India Using Spectroscopic Techniques — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
11
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.