Development and Validation of UPLC-MS / MS Method for Obtaining Favipiravir Tablet Dosage form and Evaluation of its Behavior Under forced Conditions
Süleyman Gökçe, Ayşen Höl, İbrahim Bulduk
Journal of Pharmaceutical Research International · pp. 130–140 · Published 13 Dec 2021
10.9734/jpri/2021/v33i56A33895Abstract
Aims: Favipiravir (FVP) is a drug developed against RNA viruses. It is a drug that is used actively in the treatment of coronavirus. In vitro and in vivo investigations have shown that it inhibits the virus. In this study, a recovery study of tablet formulations was carried out by developing a UPLC-MS/MS method, which is used extensively in pandemic conditions. In addition, stability studies of favipiravir agent under forced conditions were conducted. The validated method is selective, robust, simple and applicable for tablet analysis. C18 (4.6 mm × 50 mm, 2.7 μm) column was used as the stationary phase and water-methanol (80-20 v/v) containing 0.1% formic acid was used as the mobile phase. UPLC optimization; It was conducted at a wavelength of 222 nm and a flow rate of 0.8 mL/min at 40 °C, retention time was 1.155 min. The electrospray jet stream ionization source was analyzed using mass spectrometry in negative ion mode. The molecular peak for Favipiravir was [M-1] 155.9, and the daughter ion determined 112.6. The stability test method was carried out in accordance with the ICH procedure. Reaction and degradation rates of the active substance under various forced conditions (acidic, basic, oxidative, UV light and thermal conditions) were investigated. The products formed by the decomposition of the active substance under stress conditions were determined by mass spectroscopy.
Cited by 23
Aysun Dinçel, Feyyaz Onur · Drug Development and Industrial Pharmacy · 2025
Mai H. Abd El-Fattah, Yasmine A. Sharaf, Heba M. El-Sayed · BMC Chemistry · 2026
Mahendra R. Mahajan, Omkar S. Kushwaha, Subash C.B. Gopinath · Microchemical Journal · 2025
Nandini Rebbbaniboni, Seetha Mahithavani, Gayatri Somayala · International Journal of Pharmaceutical Investigation · 2023
Inas A. Abdallah, Mohammed F. El-Behairy, Rasha M. Ahmed · Chemical Papers · 2022
Swathi Naraparaju, Karuna Devi Barla, Soujanya Chaganti · Oriental Journal Of Chemistry · 2024
Yasmine Ahmed Sharaf, Mai H. Abd El-Fattah, Heba M. El-Sayed · Scientific Reports · 2023
Yasmine Ahmed Sharaf, Mai H. Abd El-Fattah, Heba M. El-Sayed · BMC Chemistry · 2023
Showing 8 of 23 known citations — external sources report more than can currently be individually listed.
Related research
- Method Development and Validation of UV-Spectrophotometric Estimation of Hydroxychloroquine Sulphate in Bulk and Pharmaceutical Dosage Form — shares topic coverage
- A New Bioanalytical Method Development & Validation for Simultaneous Estimation of Esomeprazole and Naproxen in Human Plasma by Using RP-HPLC — shares topic coverage
- Ramadan Fasting and Intracerebral Hematoma: Incidence and Outcomes — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
23
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.