Antioxidant Activity of Curcumin and Neem (Azadirachta indica) Powders: Combination Studies with ALA Using MCF-7 Breast Cancer Cells
T. K. N. Cheung, P. Singh Nigam, R. Owusu-Apenten
Journal of Applied Life Sciences International · pp. 1–12 · Published 4 Jan 2016
10.9734/JALSI/2016/22273Abstract
Introduction: Breast cancer is the most common cancer affecting women globally. The essential fatty acid a-Linolenic acid (ALA) and its oxidation products inhibit cancer cell proliferation. The effect of natural antioxidants on ALA anticancer effects has not been well characterized. Aims: To assess the effect of curcumin and neem leaf powder extract, on ALA cytotoxicity activity towards MCF-7 breast cancers. Study Design: In-vitro testing. Methodology: Antioxidant activity of neem extract and curcumin were evaluated using, four assays: Total phenolic content, Ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay or 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. Cytotoxicity activity was assessed using MCF7 cells grown in DMEM (+10% FBS) and evaluated using Sulforhodamine B colorimetric assay for cell cytotoxicity. Results: Curcumin and neem leaf extract had significant antioxidant power, and values varied between the four assays. Treatments of MCF7 cells with ALA, (0-500 µM) curcumin (0-50 µM), and neem leaf extract (0-88 µM) individually produced a concentration-dependent decrease in MCF-7 cell viability. Combination treatments using ALA with curcumin and ALA with neem were significantly less effective compared to individual treatments. Conclusion: Combinations studies indicate that the natural antioxidants curcumin and neem reduce the inhibitory effect of a-Linolenic acid towards MCF-7 breast cancer cells.
Cited by 8
M. Artiga-Artigas, A. Molet-Rodríguez, L. Salvia-Trujillo · Food and Bioprocess Technology · 2018
Nasrin DEHGHAN-NAYERI, Mina DARVISHI, Pargol MASHATI · Journal of Research in Pharmacy · 2020
María Artiga-Artigas, Yamel Lanjari-Pérez, Olga Martín-Belloso · Food Chemistry · 2018
Reem Mohammad, Subhalaxmi Roy, Bijoy Kumar Mishra · Cereal Research Communications · 2023
I Portokalakis, H Yusof, D Ghanotakis · Journal of Advances in Biology & Biotechnology · 2016
Tahrir Aldhirgham, Kathleen Henderson, Poonam Nigam · Journal of Applied Life Sciences International · 2017
Husnain Azam, Muhammad Tahir Khan, Madeeha Shahzad Lodhi · International Journal of Applied and Experimental Biology · 2023
Subendu Sarkar, Rajender Pal Singh, Gorachand Bhattacharya · 3 Biotech · 2021
Related research
- In vitro Antioxidant and Anticancer Activity of Cyanobacteria — shares topic coverage
- Review: Parkia speciosa as Valuable, Miracle of Nature — shares topic coverage
- A Selective Review of Lipid Autoxidation and Rancidity, Antioxidants, and Dithiooxamide — shares topic coverage
- Antioxidant and Haemato-Biochemical Enhancing Properties of Daucus carota in Male Wistar Rats Toxicated with Lead Chloride — shares topic coverage
- Radical Scavenging Capacity and Efficacy of Myristica fragrans (Nutmeg) Metabolites on Cladosporum herbarum of Food Origin — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
8
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.