Effect of Different Strength of Basal Media, Sucrose Concentration and Natural Additive on in-vitro Regeneration of Phalenopsis

Abstract

Phalaenopsis is a popular cut flower among orchid species. The multiplication of this orchid is very difficult through conventional breeding due to delay flowering and uneven flower characteristics. Therefore, tissue culture techniques have been extensively used for improvement of Phalaenopsis. The presence of growth regulators and the nutrients provided in the medium have a major impact on how well plant tissue culture maximizes orchid vigour. The present study was conducted to find out the effect of different strength of MS media, various concentration of sucrose and natural additive in media on invitro regeneration of Phalaenopsis. The explants were cultured on different strength of MS media (1/4, ¾, ½, and full) supplemented with 0.15, 30, 45 g L-1 sucrose with different natural additives yeast extract, tomato juice, potato extract and coconut water. After 15 weeks of culture, the results revealed that ½ strength of MS media supplanted with 30 g L-1 showed maximum percentage of explant sweeling (60.00 (54.71) ± 0.00a) and showed organogenesis (66.67 (54.71) ± 0.00a). However, the same media supplemented with ½ MS + BAP (2.5 mg/L) + NAA (0.1 mg/L) + Sucrose (30g/L) with 15% coconut water resulted maximum number of Shoots (5.22 ± 0.07), leaves (9.07 ± 0.07) and roots (3.60 ± 0.10) per explant after 30 weeks of culture. These results revealed that Phalaenopsis cultured on ½ MS media supplemented with 30 gL-1, 15% coconut water can be used for regeneration.