Characterization and Application of Aspergillus tubingensis USMI03 RPf10 Fermented Solution as a Nutrient Source for Microbial Growth

Abstract

The fermented solution of this strain contains the highest concentration of citric acid, indole acetic acid (IAA) and elements as well as phosphatase activity. Using the Aspergillus tubingensis USMI03 RPf10 fermented solution as a medium led to decrease in the growth rate of tested bacterial isolates (Bacillus subtilis, Pseudomonas fluorescens and Escherichia coli) and fungal isolates (Aspergillus sp. and Penicillium sp.) except Trichoderma viride which gave the same growth on fermented solution treatments compared to control. Whereas the tested yeast isolates (Candida olivera and Saccharomyces cerevisiae) recorded the same growth rate on fermented solution treatments as whole media and higher growth rate on fermented solution + glucose treatment, compared to control. Also, the latter treatment resulting in higher growth of some tested bacteria (B. subtilis and P. fluorescens) and the same growth rate of other bacteria (E. coli), compared to synthetic media. The growth of Rhizopus sp. decreased on fermented solution treatments than control. It is suggested that, using A. tubingensis USMI03 RPf10 fermented solution as a whole medium or as a mineral could be used as a source for microbial growth which varied from one microorganism to another.