Detection of Phytotoxin Produced from Leaf, Neck and Finger Blast Disease Causing Magnaporthe grisea through GC-MS Analysis

Abstract

Finger millet blast is the most devastating disease affecting different aerial parts of the plant at all growth stages starting from seedling to grain formation. Magnaporthe grisea being a ubiquitous pathogen with many hosts, understand the basis for host pathogen interaction and role of toxins will be useful in development of resistant varieties and their screening procedures. In this study, the presence of toxic volatile compounds were detected  by Thin Layer Chromatography (TLC) in the culture filtrate of leaf blast, neck blast and finger blast pathogen at different bands indicating various retention factor (Rf). Likewise the several toxic compounds were detected from M. grisea through GC-MS analysis from the semi-purified crude toxin in vitro viz., 1-Hexadecene (CAS), 1-Octadecene (CAS), Quinic acid,  1,2-Benzenedicarboxylic acid- bis (2-ethylhexyl) ester (CAS) from leaf blast isolate, Hippocasine, Holothurinogenin-2, Neophytadiene, 1,2-Benzene-dicarboxylic acid, diisooctyl ester (CAS), à-Patchoulene (CAS),  Synaptogenin B, 1-Naphthalenol, decahydro-1,4a-dimethyl-7-(1-methylethylidene),  Zingiberene (CAS) from neck blast isolate and 2H-Pyran-2-one, 6-hexyltetrahydro-delta-Hexylvalerolactone from finger blast isolate were detected.