The Maturity of Female Gonads of G2 Transgenic Mutiara Catfish (Clarias sp.)

Abstract

The G2 trangsenic Mutiara catfish (Clarias sp.) (MTG) is a Mutiara catfish that is inserted with the CgGH gene (Clarias gariepinus Growth Hormone) through the transgenesis. The effect of transgenesis stimulates gonad growth of G2 transgenic Mutiara catfish (Clarias sp.) faster than non-transgenic fish. Study aimed to analyze the maturity of the gonads and the spawning ability of female G2 transgenic Mutiara catfish (Clarias sp.) to obtain superior broodstock candidates. Experimental method with completely randomized design (pair of parents used as treatment and repeated four times) for spawning was used for this study. Three pairs of parent G2 were crossed semi-artificially as treatment A (female 1 MTG G2 crossed with male 1 MTG G2), B (female 2 MTG G2 crossed with male 2 MTG G2) and C (female 3 MTG G2 crossed with male 3 MTG G2). The results showed that the performance of female G2 transgenic Mutiara catfish (Clarias sp.) (treatment A, B and C) was higher given non-transgenic with an average relative fecundity of 82,438 eggs / kg of broodstock, an average egg diameter of 1.76 mm and an average egg weight 1.75 mg. These indications suggest that GH transgenesis increases gonadal maturity. The gonad maturity profile reached the stage of complete maturity (full ripe) compared to Sangkuriang catfish (Clarias gariepinus) (immature gonads). Therefore it is necessary to compare the reproductive performance of G2 transgenic Mutiara catfish (Clarias sp.) with non-transgenic fish (Sangkuriang) as candidates for superior broodstock of catfish.