Molecular Characterization and Antibiotic Resistance Profile of Bacteria Associated with Brycinus longipinnis from Eggua Station on Yewa River

Abstract

Fish is one of the most highly perishable food products, during handling and storage, causing rapid quality deterioration of fresh fish and limits the shelf life of the product. Bacteria isolates were collected from the gills, skins and guts of Brycinus longipinnis from Eggua, station on Yewa River. The bacteria isolates were assessed using 16S rRNA gene sequencing method to identify them and to construct the phylogenetic relationship. Sixteen bacteria isolates were selected, their  morphological characteristics were determined, the DNA of the isolates were extracted using CTAB method, PCR amplification of 16S ribosomal RNA gene of isolates was carried out using universal primer for bacteria, purification of the PCR product was done using ethanol precipitation, later it was sequenced using an automated DNA sequencer. The sequence data were compared with other gene sequences in GenBank database (NCBI) using a BLAST search to find closely related sequences. Eight different bacteria strains were identified from skins, gills and guts of Brycinus longipinnis. The strains are Pseudomonas spp (15%), Comamonas spp (10%), Escherichia coli (10%), Proteus spp (10%), Entrobacter spp (10%), Pseudoalteromonas spp (5%), Alcaligenes  faecalis (5%), and Serratia marcescens (5%) frequency of bacteria. Most of the strains from Brycinus longipinnis were sensitive to Ofloxacin and Ciprofloxacin antibiotics; and resistant to Cefuroxime and Cefixime. It can therefore be concluded that Brycinus longipinnis harbors different species of bacteria.