Crude and Chitosan Nano-particles Extracts of Some Maggots as Antioxidant and Anticancer Agents

Abstract

The antioxidant effects besides anticancer activities of Musca domestica, Lucilia sericata and Chrysomya albiceps maggots extracts against human liver carcinoma (HepG-2) and human colon carcinoma (HCT-116) were investigated. Two kinds of extracts, crude and chitosan nanoparticles (CNPs) were prepared. The antioxidant activity of different tested extracts was performed by DPPH radical scavenging method, the results obtained revealed that, the highest levels of DPPH scavenging activity were exhibited by the crude extracts of tested maggots with preference to C. albiceps extract, which exhibited a much more potent activity followed by L. sericata and M. domestica in crude and CNPs extracts. Crude extracts have lower anticancer activity than the CNPs extracts; however, the lowest percentage of cell viability (6.7±0.7%) was recorded by L. sericata crude extract against HCT-116, followed by C. albiceps crude extract (7.57±1.25%) against HepG-2 at the highest used concentration 100 µg/ml. The strongest anticancer activity was observed with CNPs extracts and it was recorded at concentrations of 80, 90 and 100 µg/ml against cell lines tested. Depending on Median inhibitory concentrations (IC50) of maggots crude and CNPs extracts, the IC50 values were in the range of 37.3 to 74.3 µg/ml and the highest anticancer activity was obtained by C. albiceps CNPs extracts against cell lines tested. In conclusion, both tested extracts have optimistic antioxidant activity. CNPs extracts have great therapeutic potential due to its anticancer inducing activities.