An Application of Residue Number System (RNS) to a Next-Generation Sequencing - SOLiD

Abstract

Aims: This research work leverages the possibility and potential of an RNS-dibase table to generate the sequence primer and colour space for successful SOLiD sequencing. This design is flexible as compared with its binary counterpart and also presents a quaternary approach to SOLiD sequencing. Study Design: RNS sequence primer and colour space are generated resulting in a successful RNS-SOLiD Sequencing. Methodology: One of the most accurate Next Generation Sequencing (NGS) methods currently in use is Sequencing by Oligonucleotide Ligation and Detection (SOLiD). It combines ligation-base chemistry with a di-base labelled probe to produce an accuracy rate of about 99.9999%. RNS has the potential of generating the di-base table which is the Rosetta stone for SOLiD sequencing. Leveraging this possibility, the sequence primer and colour space which are requirements for a successful SOLiD sequencing are generated in RNS space. Following this, SOLiD sequencing is therefore designed using RNS. Results: An RNS di-base table is presented and this serves as a look-up table for the generation of RNS sequence primer and colour space for successful SOLiD sequencing. A platform-independent algorithm is also developed that effectively illustrates SOLiD sequencing in RNS space. Conclusion: This lays the groundwork for the incorporation of RNS into SOLiD sequencing. This design is flexible and buttresses the quest for a quaternary number system for molecular biological design and analysis.