Skip to content
Research Article Open access CC BY 4.0

HPLC-UV Standard Phenolic Constituents of African Bush Mango (Irvingia gabonensis) and Molecular Docking on Polyphenol Oxidases

Catherine Joke Adeseko, David Morakinyo Sanni, Sule Ola Salawu, Ige Joseph Kade, Toluwase Hezekiah Fatoki

Journal of Applied Life Sciences International · pp. 1–11 · Published 4 Oct 2019

10.9734/jalsi/2019/v22i130119

Abstract

Introduction: Irvingia gabonensis belongs to the Irvingiaceae plant family and commonly known as the African bush mango, wild mango, Dika nut and Manguier sauvage. The fruits of I. gabonensis are edible and their use in traditional medicine has been reported for the treatment of diabetes, diarrhea, wound ulcer, hepatic disorder, microbial infections, and inflammatory pains.  Aim: This study aimed to identify the standard phenolic contents of I. gabonensis fruit pulp and peel extracts and investigate the bind energy on polyphenol oxidase in order to know why browning of I. gabonensis fruit is often rapidly occur and how to prevent it with suitable inhibitor in an industrial processing. Results: The phenolics that were identified include ellagic acid, cinnamic acid, gallic acid, 3-friedelanone, lupeol, kaempferol, quercetin, alpha-curcumene and zingiberene. The two PPO from Solanum lycopersicum (PDB ID: 6HQI) and Mangifera indica (PDB: D2XZ13_model) have different binding energies across the ligands with highest score by 3-friedelanone (-14.3 and -16.1 kcal/mol respectively), followed by lupeol (-13.9 and -14.6 kcal/mol respectively). The differences in the binding energies across the plant PPOs could be due to variation in the amino acid composition and more importantly the amino acid residues that participate in the catalytic and allosteric activities Conclusion: This study has shown the reason behind rapid browning that usually occur in I. gabonensis and more importantly, the need for effective edible inhibitors of PPO.

Irvingia gabonensis African bush mango HPLC-UV phenolic constituents molecular docking

References (24)

  1. 1 SWISS-MODEL: homology modelling of protein structures and complexes [DOI]
  2. 2 HHblits: lightning-fast iterative protein sequence searching by HMM-HMM alignment [DOI]
  3. 3 PHYSICOCHEMICAL PROPERTIES AND FUNCTION OF PLANT POLYPHENOL OXIDASE: A REVIEW [DOI]
  4. 4 Copper–O2 reactivity of tyrosinase models towards external monophenolic substrates: molecular mechanism and comparison with the enzyme [DOI]
  5. 5 Polyphenol Oxidases in Crops: Biochemical, Physiological and Genetic Aspects [DOI]
  6. 6 Separation, Identification, Quantification, and Method Validation of Anthocyanins in Botanical Supplement Raw Materials by HPLC and HPLC−MS [DOI]
  7. 7 Quinone Reductase Induction as a Biomarker for Cancer Chemoprevention [DOI]
  8. 8 Characterization of polyphenol oxidase from Cape gooseberry (Physalis peruviana L.) fruit [DOI]
  9. 9 Relationship between Apple Ripening and Browning: Changes in Polyphenol Content and Polyphenol Oxidase [DOI]
  10. 10 Biochemical characterization and process stability of polyphenoloxidase extracted from Victoria grape (Vitis vinifera ssp. Sativa) [DOI]
  11. 11 Extraction and partial characterization of polyphenol oxidase from banana (Musa acuminata Grande naine) roots [DOI]
  12. 12 Analgesic effect of Irvingia gabonensis stem bark extract [DOI]
  13. 13 IGOB131, a novel seed extract of the West African plant Irvingia gabonensis, significantly reduces body weight and improves metabolic parameters in overweight humans in a randomized double-blind placebo controlled investigation [DOI]
  14. 14 Polyphonel Oxidases: Biochemical and Molecular Characterization, Distribution, Role and its Control [DOI]
  15. 15 Studies on Aldose Reductase Inhibitors from Natural Products. IV. Constituents and Aldose Reductase Inhibitory Effect of Chrysanthemum morifolium, Bixa orellana and Ipomoea batatas. [DOI]
  16. 16 Characterization of Polyphenol Oxidase Activity in Juices from 12 Underutilized Tropical Fruits with High Agroindustrial Potential [DOI]
  17. 17 Purification and characterization of polyphenol oxidase from purslane [DOI]
  18. 18 Ultra High-Performance Liquid Chromatography with High-Resolution Mass Spectrometry Analysis of African Mango (Irvingia gabonensis) Seeds, Extract, and Related Dietary Supplements [DOI]
  19. 19 Friedelanone and other triterpenoids from hymenocardia acida [DOI]
  20. 20 In vitro hepatoprotective and antioxidant activities of the crude extract and isolated compounds from Irvingia gabonensis
  21. 21 Polyphenol oxidase from sweet potato: purification and properties [DOI]
  22. 22 Hypoglycaemic Effects of Viscous Preparation ofIrvingia gabonensis(Dikanut) Seeds in Streptozotocin-Induced Diabetic Wistar Rats [DOI]
  23. 23 Selected Properties of Polyphenol Oxidase Obtained from Ispir Sugar Bean [DOI]
  24. 24 Polyphenol content and in vitro antioxidant activity of methanol extract of seeds of Irvingia gabonensis Baill of Nigerian origin.

Cited by 13

Anti-diabetic, anti-pancreatic lipase, and anti-protein glycation potential of Irvingia gabonensis stem bark extracts: in vitro and in silico studies

Akhere A. Omonkhua, Catherine Otitolaiye, Bobby Aguebor-Ogie · In Silico Pharmacology · 2024

Biochemical studies of enzyme-induced browning of African bush mango (Irvingia gabonensis) fruit pulp

Catherine Joke Adeseko, David Morakinyo Sanni, Olusola Tosin Lawal · Preparative Biochemistry & Biotechnology · 2021

Network analysis, sequence and structure dynamics of key proteins of coronavirus and human host, and molecular docking of selected phytochemicals of nine medicinal plants

Toluwase Hezekiah Fatoki, Omodele Ibraheem, Ibukun Oladejo Ogunyemi · Journal of Biomolecular Structure and Dynamics · 2020

Irvingia gabonensis baill. (African Mango): A comprehensive review of its ethnopharmacological significance, unveiling its long-standing history and therapeutic potential

Yassmin Raafat Hassan, Riham A. El-Shiekh, Hala Mohamed El Hefnawy · Journal of Ethnopharmacology · 2024

Showing 4 of 13 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

64

Page views

0

PDF downloads

28

Outbound clicks

13

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.