Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Chemical Constituents
Graeme Kirkpatrick, Poonam S. Nigam, Richard Owusu-Apenten
Journal of Advances in Biology & Biotechnology · pp. 1–7 · Published 6 Nov 2017
10.9734/JABB/2017/37242Abstract
Aims: To compare the total phenol content, antioxidant capacity and antibacterial activity of methyl syringate (MSY), methylglyoxal (MGO) and phenyllactic acid (PLA) as major components from Manuka honey Study Design: In-vitro study. Place and Duration of Study: Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, University of Ulster, Coleraine Campus, between June 2016 and September 2017. Methodology: Total phenols content (TPC) was determined using the Folin-Ciocalteu assay. Antioxidant capacity was evaluated as 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical quenching activity or iron (III) reducing antioxidant capacity (IRAC). Antibacterial activity was measured using the disc diffusion assay with, E.coli, Bacillus subtilis or Staphylococcus aureus. Results: The TPC for MSY was 60.8 % gallic acid equivalents (GAE) and significantly higher than 1.6-3.2% GAE observed for MGO or PLA. The antioxidant capacity for MSY (128% to 270% trolox equivalents (TE)) was significantly higher compared with -6% to 4.4%TE for MGO or PLA. A disc diffusion assay for MGO and PLA showed antibacterial power but MSY had no antibacterial activity. Conclusion: Methylglyoxal and PLA from Manuka honey showed antibacterial activity but no detectable antioxidant and total phenol character. Methyl syringate, which shows high antioxidant capacity and TPC, had no detectable antibacterial activity. Total phenols content and antioxidant power of Manuka honey is unlikely to be related to its antibacterial activity.
Cited by 11
Hau Ching Wan, Bushra Sultana, Poonam Singh Nigam · Beverages · 2018
Pauline McLoone, Toheeb Olalekan Oladejo, Laura Kassym · Phytotherapy Research · 2024
Xiaoling Zhou, Wei Zhao, Zihong Ye · Foods · 2025
Nurul Ainaa Farhanah Mat Ramlan, Ezzat Mohamad Azman, Kharidah Muhammad · Journal of the Science of Food and Agriculture · 2023
Pauline McLoone, Dina Tabys, Sofiya Yunussova · Natural Product Research · 2022
Caoimhin Mackin, Divakar Dahiya, Poonam Singh Nigam · Pharmaceuticals · 2023
N. V. Makarova, D. F. Ignatova · Innovations and Food Safety · 2020
Laura Kassym, Assiya Kussainova, Yuliya Semenova · Pharmaceuticals · 2024
Sushil Anand, Margaret Deighton, George Livanos · Scientific Reports · 2019
Youssef Elamine, Hamada Imtara, Maria Graça Miguel · Applied Sciences · 2021
Related research
- In vitro Antioxidant and Anticancer Activity of Cyanobacteria — shares topic coverage
- Review: Parkia speciosa as Valuable, Miracle of Nature — shares topic coverage
- A Selective Review of Lipid Autoxidation and Rancidity, Antioxidants, and Dithiooxamide — shares topic coverage
- Antioxidant and Haemato-Biochemical Enhancing Properties of Daucus carota in Male Wistar Rats Toxicated with Lead Chloride — shares topic coverage
- Radical Scavenging Capacity and Efficacy of Myristica fragrans (Nutmeg) Metabolites on Cladosporum herbarum of Food Origin — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
11
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.